human CYP27B1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human CYP27B1.
shAAV-206470
AAV-h-CYP27B1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human CYP27B1 (cytochrome P450 family 27 subfamily B member 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR
Description (Vector)
CYP27B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.
Gene ID
1594
Gene Name (Vector)
cytochrome P450 family 27 subfamily B member 1
Gene Symbol
CYP27B1
HGNC ID
HGNC:2606
ORF Size (aa)
1527
ORF Size (bp)
1527 bp
RefSeq ID
NM_000785
RefSeq Synonyms
NM_000785, BC136386, BC001776,
Species
human
UniGene ID
Hs.524528
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.