human CYBB shRNA silencing AAV

Name
AAV-h-CYBB-shRNA

Cat No
shAAV-206408

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CYBB.

shAAV-206408

AAV-h-CYBB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CYBB (cytochrome b-245 beta chain). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX

Description (Vector)

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

Gene ID

1536

Gene Name (Vector)

cytochrome b-245 beta chain

Gene Symbol

CYBB

HGNC ID

HGNC:2578

ORF Size (aa)

1713

ORF Size (bp)

1713 bp

RefSeq ID

NM_000397

RefSeq Synonyms

NM_000397, BC032720,

Species

human

UniGene ID

Hs.292356

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.