human COL9A1 shRNA silencing AAV

Name
AAV-h-COL9A1-shRNA

Cat No
shAAV-205720

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human COL9A1.

shAAV-205720

AAV-h-COL9A1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human COL9A1 (collagen type IX alpha 1 chain). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

DJ149L1.1.2; EDM6; MED; STL4

Description (Vector)

COL9A1 encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene.

Gene ID

1297

Gene Name (Vector)

collagen type IX alpha 1 chain

Gene Symbol

COL9A1

HGNC ID

HGNC:2217

ORF Size (aa)

2037

ORF Size (bp)

2037 bp

RefSeq ID

NM_001851

RefSeq Synonyms

NM_078485, NM_001851, BC063646, BC015409,

Species

human

UniGene ID

Hs.590892

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.