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human COL5A2 shRNA silencing AAV

Name 
AAV-h-COL5A2-shRNA
Cat No 
shAAV-205711
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human COL5A2.

shAAV-205711
AAV-h-COL5A2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human COL5A2 (collagen type V alpha 2 chain). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
EDSC
Description (Vector)
COL5A2 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A2 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Gene ID
1290
Gene Name (Vector)
collagen type V alpha 2 chain
Gene Symbol
COL5A2
HGNC ID
HGNC:2210
ORF Size (aa)
3420
ORF Size (bp)
3420 bp
RefSeq ID
NM_000393
RefSeq Synonyms
NM_000393, BC086874,
Species
human
UniGene ID
Hs.445827

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.