human COL1A1 shRNA silencing AAV

Name
AAV-h-COL1A1-shRNA

Cat No
shAAV-205689

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human COL1A1.

shAAV-205689

AAV-h-COL1A1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human COL1A1 (collagen type I alpha 1 chain). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

EDSC; OI1; OI2; OI3; OI4

Description (Vector)

COL1A1 encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. (provided by R. Dalgleish, Feb 2008)

Gene ID

1277

Gene Name (Vector)

collagen type I alpha 1 chain

Gene Symbol

COL1A1

HGNC ID

HGNC:2197

ORF Size (aa)

4395

ORF Size (bp)

4395 bp

RefSeq ID

NM_000088

RefSeq Synonyms

NM_000088, BC036531,

Species

human

UniGene ID

Hs.681002

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.