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human CHD3 shRNA silencing AAV

Name 
AAV-h-CHD3-shRNA
Cat No 
shAAV-205113
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CHD3.

shAAV-205113
AAV-h-CHD3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CHD3 (chromodomain helicase DNA binding protein 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATP-dependent helicase CHD3; CHD-3; chromodomain-helicase-DNA-binding protein 3; hZFH; mi-2 autoantigen 240 kDa protein; Mi-2a; Mi2-ALPHA; ZFH; zinc finger helicase; zinc-finger helicase (Snf2-like)
Description (eCommerce)
CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.
Description (Vector)
CHD3 encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Gene ID
1107
Gene Name (eCommerce)
chromodomain helicase DNA binding protein 3
Gene Name (Vector)
chromodomain helicase DNA binding protein 3
Gene Symbol
CHD3
HGNC ID
HGNC:1918
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
6135
ORF Size (bp)
6135 bp
Protein Name (eCommerce)
Chromodomain-helicase-DNA-binding protein 3
RefSeq ID
NM_001005271
RefSeq Synonyms
NM_005852, NM_001005273, NM_001005271, BC156472,
Research Areas
Cell Cycle,Chromatin Remodeling,Cytoskeleton,Helicase Activity,Transcription Factor/Regulator,Zinc-finger
Research Areas (Faceted)
cell_cycle,transcription_translation,genetics,cell_biology
Species
human
Target Sentence
CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.
UniGene ID
Hs.25601
UniProt ID (eCommerce)
Q12873

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.