human CHD1L shRNA silencing AAV

Name
AAV-h-CHD1L-shRNA

Cat No
shAAV-205111

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human CHD1L.

shAAV-205111

AAV-h-CHD1L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CHD1L (chromodomain helicase DNA binding protein 1 like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

80

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ALC1; amplified in liver cancer 1; amplified in liver cancer protein 1; CHDL; chromodomain-helicase-DNA-binding protein 1-like

Description (eCommerce)

Chromodomain-helicase-DNA-binding protein 1-like (CHD1L) is a DNA helicase which plays a role in chromatin-remodeling following DNA damage. CHD1L is targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. It is able to catalyze nucleosome sliding in an ATP-dependent manner, and its helicase activity is strongly stimulated upon poly(ADP-ribose)-binding [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86WJ1].

Description (Vector)

CHD1L encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Several alternatively spliced transcripts variants have been described for this gene.

Gene ID

9557

Gene Name (eCommerce)

chromodomain helicase DNA binding protein 1 like

Gene Name (Vector)

chromodomain helicase DNA binding protein 1 like

Gene Symbol

CHD1L

HGNC ID

HGNC:1916

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2082

ORF Size (bp)

2082 bp

Protein Name (eCommerce)

Chromodomain-helicase-DNA-binding protein 1-like

RefSeq ID

NM_004284

RefSeq Synonyms

NM_024568, NM_004284, NM_001256338, NM_001256337, NM_001256336, BC077717,

Research Areas

Chromatin Remodeling,DNA Damage/Repair,Helicase Activity

Research Areas (Faceted)

cell_biology,genetics,transcription_translation

Species

human

Target Sentence

Chromodomain-helicase-DNA-binding protein 1-like (CHD1L) is a DNA helicase which plays a role in chromatin-remodeling following DNA damage. CHD1L is targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. It is able to catalyze nucleosome sliding in an ATP-dependent manner, and its helicase activity is strongly stimulated upon poly(ADP-ribose)-binding [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86WJ1].

UniGene ID

Hs.191164

UniProt ID (eCommerce)

Q86WJ1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.