human CH25H shRNA silencing AAV

Name
AAV-h-CH25H-shRNA

Cat No
shAAV-205084

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CH25H.

shAAV-205084

AAV-h-CH25H-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CH25H (cholesterol 25-hydroxylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

C25H

Description (Vector)

This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.

Gene ID

9023

Gene Name (Vector)

cholesterol 25-hydroxylase

Gene Symbol

CH25H

HGNC ID

HGNC:1907

ORF Size (aa)

819

RefSeq ID

NM_003956

RefSeq Synonyms

NM_003956, BC072430, BC017843,

Species

human

UniGene ID

Hs.47357

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.