human CDC25A shRNA silencing AAV

Name 
AAV-h-CDC25A-shRNA
Cat No 
shAAV-204736
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CDC25A.

shAAV-204736
AAV-h-CDC25A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CDC25A (cell division cycle 25A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CDC25 isoform A1-CAG; CDC25A2; CDC25A2-CAG isoform; dual specificity phosphatase CDC25A; M-phase inducer phosphatase 1
Description (eCommerce)
CDC25a is a member of the CDC25 family of protein phosphatases. CDC25 is involved in promoting the G1 to S phase as well as the G2 to M phase transition of the cell cycle by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases (CDKs). CDC25a is an oncogene and has been found to be overexpressed in a subset of human cancers.
Description (Vector)
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
993
Gene Name (eCommerce)
cell division cycle 25A
Gene Name (Vector)
cell division cycle 25A
Gene Symbol
CDC25A
HGNC ID
HGNC:1725
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1575
ORF Size (bp)
1575 bp
Protein Name (eCommerce)
M-phase inducer phosphatase 1
RefSeq ID
NM_001789
RefSeq Synonyms
NM_201567, NM_001789, BC018642, BC007401,
Research Areas
Breast Cancer,Cancer,Cell Cycle,Cell Division,DNA Damage/Repair,DNA Replication,Kinase/Phosphatase,MicroRNAs in Cancer,Phosphatase,Prostate Cancer,Replication,Signal Transduction,TGF-beta Signaling
Research Areas (Faceted)
cancer,genetics,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,signal_transduction,immunology
Species
human
Target Sentence
CDC25a is a member of the CDC25 family of protein phosphatases. CDC25 is involved in promoting the G1 to S phase as well as the G2 to M phase transition of the cell cycle by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases (CDKs). CDC25a is an oncogene and has been found to be overexpressed in a subset of human cancers.
UniGene ID
Hs.437705
UniProt ID (eCommerce)
P30304

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.