human B3GALT5 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human B3GALT5.
shAAV-201877
AAV-h-B3GALT5-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human B3GALT5 (beta-1,3-galactosyltransferase 5). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
3-GalTase 5; B3GalT-V; B3GalTx; B3T5; beta-1; beta-3-Gx-T5; beta3Gal-T5; GLCT5
Description (Vector)
B3GALT5 is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). B3GALT5 encodes the most probable candidate for synthesis of the type 1 Lewis antigens which are frequently found to be elevated in gastrointestinal and pancreatic cancers. The encoded protein is inactive with N-linked glycoproteins and functions in mucin glycosylation. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.
Gene ID
10317
Gene Name (Vector)
beta-1,3-galactosyltransferase 5
Gene Symbol
B3GALT5
HGNC ID
HGNC:920
ORF Size (aa)
933
ORF Size (bp)
933 bp
RefSeq ID
NM_033170
RefSeq Synonyms
NM_033173, NM_033172, NM_033171, NM_033170, NM_006057, NM_001278650, BC104864, BC104862,
Species
human
UniGene ID
Hs.655094
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.