human ATP6V1D shRNA silencing AAV

Name 
AAV-h-ATP6V1D-shRNA
Cat No 
shAAV-201762
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ATP6V1D.

shAAV-201762
AAV-h-ATP6V1D-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ATP6V1D (ATPase H+ transporting V1 subunit D). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATP6M; VATD; VMA8
Description (Vector)
ATP6V1D encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. ATP6V1D encodes the V1 domain D subunit protein.
Gene ID
51382
Gene Name (Vector)
ATPase H+ transporting V1 subunit D
Gene Symbol
ATP6V1D
HGNC ID
HGNC:13527
ORF Size (aa)
744
ORF Size (bp)
744 bp
RefSeq ID
NM_015994
RefSeq Synonyms
NM_015994, BC031002, BC025373, BC001411,
Species
human
UniGene ID
Hs.272630

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.