human ATP5E shRNA silencing AAV

Name
AAV-h-ATP5E-shRNA

Cat No
shAAV-201716

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ATP5E.

shAAV-201716

AAV-h-ATP5E-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ATP5E (ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ATPE; MC5DN3

Description (Vector)

ATP5E encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5E encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.

Gene ID

514

Gene Name (Vector)

ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit

Gene Symbol

ATP5E

HGNC ID

HGNC:838

ORF Size (aa)

156

ORF Size (bp)

156 bp

RefSeq ID

NM_006886

RefSeq Synonyms

NM_006886, NM_001001977, BC105811, BC003671, BC001690,

Species

human

UniGene ID

Hs.177530

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.