human ARMET shRNA silencing AAV

Name 
AAV-h-ARMET-shRNA
Cat No 
shAAV-201445
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MANF.

shAAV-201445
AAV-h-ARMET-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MANF (mesencephalic astrocyte derived neurotrophic factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Arginine-rich protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET
Description (eCommerce)
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression MANF increases susceptibility to ER stress-induced death and results in cell proliferation. MANF activity is important in promoting the survival of dopaminergic neurons [taken from NCBI Entrez Gene (Gene ID: 7873)].
Description (Vector)
MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related.
Gene ID
7873
Gene Name (eCommerce)
mesencephalic astrocyte derived neurotrophic factor
Gene Name (Vector)
mesencephalic astrocyte derived neurotrophic factor
Gene Symbol
MANF
HGNC ID
HGNC:15461
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
558
Protein Name (eCommerce)
Mesencephalic astrocyte-derived neurotrophic factor
RefSeq ID
NM_006010
RefSeq Synonyms
NM_006010, BC113590, BC113588, BC007282,
Research Areas
Protein Folding,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology
Species
human
Target Sentence
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression MANF increases susceptibility to ER stress-induced death and results in cell proliferation. MANF activity is important in promoting the survival of dopaminergic neurons [taken from NCBI Entrez Gene (Gene ID: 7873)].
UniGene ID
Hs.436446
UniProt ID (eCommerce)
P55145

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.