human ARHGEF12 shRNA silencing AAV

Name 
AAV-h-ARHGEF12-shRNA
Cat No 
shAAV-201357
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ARHGEF12.

shAAV-201357
AAV-h-ARHGEF12-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ARHGEF12 (Rho guanine nucleotide exchange factor 12). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
LARG; leukemia-associated rho guanine nucleotide exchange factor; leukemia-associated RhoGEF; PRO2792; Rho guanine nucleotide exchange factor (GEF) 12; rho guanine nucleotide exchange factor 12
Description (eCommerce)
LARG (leukemia-associated RhoGEF) is homologous to PDZ-RhoGEF and functions as a RhoA-specific guanine nucleotide exchange factor. RhoGEFs catalyze the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. LARG contains a PDZ [post synaptic density protein (PSD95), Drosophila discs-large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. LARG is proposed to play a role in cell polarity and microtubule dynamics and may act as a GTPase-activating protein (GAP) for the heterotrimeric Galpha(12) and Galpha(13) proteins.
Description (Vector)
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
23365
Gene Name (eCommerce)
Rho guanine nucleotide exchange factor 12
Gene Name (Vector)
Rho guanine nucleotide exchange factor 12
Gene Symbol
ARHGEF12
HGNC ID
HGNC:14193
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4632
ORF Size (bp)
4632 bp
Protein Name (eCommerce)
Rho guanine nucleotide exchange factor 12
RefSeq ID
NM_015313
RefSeq Synonyms
NM_015313, NM_001301084, NM_001198665, BC152900,
Research Areas
Apoptosis,Axon Guidance,Cancer,Cardiovascular,Cytoskeleton,GPCR Signaling,Neurobiology,NGF Signaling,Rho Signaling,Signal Transduction
Research Areas (Faceted)
cardiovascular,cancer,cell_biology,neurobiology,signal_transduction
Species
human
Target Sentence
LARG (leukemia-associated RhoGEF) is homologous to PDZ-RhoGEF and functions as a RhoA-specific guanine nucleotide exchange factor. RhoGEFs catalyze the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. LARG contains a PDZ [post synaptic density protein (PSD95), Drosophila discs-large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. LARG is proposed to play a role in cell polarity and microtubule dynamics and may act as a GTPase-activating protein (GAP) for the heterotrimeric Galpha(12) and Galpha(13) proteins.
UniGene ID
Hs.24598
UniProt ID (eCommerce)
Q9NZN5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.