human ARHGEF1 shRNA silencing AAV

Name 
AAV-h-ARHGEF1-shRNA
Cat No 
shAAV-201352
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ARHGEF1.

shAAV-201352
AAV-h-ARHGEF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ARHGEF1 (Rho guanine nucleotide exchange factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
115 kDa guanine nucleotide exchange factor; 115-kD protein; GEF1; LBCL2; LSC; Lsc homolog; p115RhoGEF; P115-RHOGEF; Rho guanine nucleotide exchange factor (GEF) 1; rho guanine nucleotide exchange factor 1; SUB1.5
Description (eCommerce)
P115-RhoGEF plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. P115-RhoGEF acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. The gene name for p115-RhoGEF is ARHGEF1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92888].
Description (Vector)
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Gene ID
9138
Gene Name (eCommerce)
Rho guanine nucleotide exchange factor 1
Gene Name (Vector)
Rho guanine nucleotide exchange factor 1
Gene Symbol
ARHGEF1
HGNC ID
HGNC:681
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2847
ORF Size (bp)
2847 bp
Protein Name (eCommerce)
Rho guanine nucleotide exchange factor 1
RefSeq ID
NM_004706
RefSeq Synonyms
NM_199002, NM_198977, NM_004706, BC067262, BC034013, BC011726,
Research Areas
Axonogenesis,Cancer,Cardiovascular,Cytoskeleton,EGFR Signaling,GPCR Signaling,Neurobiology,NGF Signaling,RAC1 Signaling,Rho Signaling,Signal Transduction
Research Areas (Faceted)
cardiovascular,cancer,signal_transduction,neurobiology,developmental_biology,cell_biology,signal_transduction,neurobiology
Species
human
Target Sentence
P115-RhoGEF plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. P115-RhoGEF acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. The gene name for p115-RhoGEF is ARHGEF1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92888].
UniGene ID
Hs.631550
UniProt ID (eCommerce)
Q92888

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.