human APPL2 shRNA silencing AAV

Name
AAV-h-APPL2-shRNA

Cat No
shAAV-201224

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human APPL2.

shAAV-201224

AAV-h-APPL2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human APPL2 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

DIP13B

Description (Vector)

APPL2 is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome.

Gene ID

55198

Gene Name (Vector)

adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2

Gene Symbol

APPL2

HGNC ID

HGNC:18242

ORF Size (aa)

1995

ORF Size (bp)

1995 bp

RefSeq ID

NM_018171

RefSeq Synonyms

NM_018171, NM_001251905, NM_001251904, BC033731,

Species

human

UniGene ID

Hs.506603

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.