human ALPL shRNA silencing AAV

Name 
AAV-h-ALPL-shRNA
Cat No 
shAAV-200808
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ALPL.

shAAV-200808
AAV-h-ALPL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ALPL (alkaline phosphatase, liver/bone/kidney). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AP-TNAP; APTNAP; HOPS; TNAP; TNSALP
Description (Vector)
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.
Gene ID
249
Gene Name (Vector)
alkaline phosphatase, liver/bone/kidney
Gene Symbol
ALPL
HGNC ID
HGNC:438
ORF Size (aa)
1575
ORF Size (bp)
1575 bp
RefSeq ID
NM_000478
RefSeq Synonyms
NM_001177520, NM_001127501, NM_000478, BC136325, BC126165, BC110909, BC090861, BC066116, BC021289,
Species
human
UniGene ID
Hs.75431

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.