human AKAP8 shRNA silencing AAV

Name 
AAV-h-AKAP8-shRNA
Cat No 
shAAV-200666
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human AKAP8.

shAAV-200666
AAV-h-AKAP8-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human AKAP8 (A-kinase anchoring protein 8). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A kinase (PRKA) anchor protein 8; AKAP 95; AKAP-8; AKAP95; AKAP-95; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; A-kinase anchor protein, 95kDa
Description (eCommerce)
AKAP8/AKAP95 has been characterized as a 95 kDa protein kinase A (PKA)-anchoring protein and regulatory subunit of PKA that functions to recruit the condensin complex for chromosome condensation during mitosis. A role in DNA replication has also been demonstrated through its interaction with the pre-replication complex component, MCM2 (mini-chromosome maintenance 2). Inhibition of AKAP8/AKAP95 association with MCM2 inhibits replication initiation and elongation in vitro. AKAP8/AKAP95 has also been shown to be involved in apoptosis. KAP8/AKAP95 associates with caspase 3 and serves as a carrier to transport caspase 3 from the cytoplasm to the nucleus to induce morphological changes in the nucleus.
Description (Vector)
AKAP8 encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. AKAP8 encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.
Gene ID
10270
Gene Name (eCommerce)
A-kinase anchoring protein 8
Gene Name (Vector)
A-kinase anchoring protein 8
Gene Symbol
AKAP8
HGNC ID
HGNC:378
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2079
ORF Size (bp)
2079 bp
Protein Name (eCommerce)
A-kinase anchor protein 8
RefSeq ID
NM_005858
RefSeq Synonyms
NM_005858, BC037270,
Research Areas
Cell Cycle,Golgi Apparatus,Immunology,Mitochondrion,Signal Transduction,TNF Signaling,Transport,Zinc-finger
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,immunology,signal_transduction
Species
human
Target Sentence
AKAP8/AKAP95 has been characterized as a 95 kDa protein kinase A (PKA)-anchoring protein and regulatory subunit of PKA that functions to recruit the condensin complex for chromosome condensation during mitosis. A role in DNA replication has also been demonstrated through its interaction with the pre-replication complex component, MCM2 (mini-chromosome maintenance 2). Inhibition of AKAP8/AKAP95 association with MCM2 inhibits replication initiation and elongation in vitro. AKAP8/AKAP95 has also been shown to be involved in apoptosis. KAP8/AKAP95 associates with caspase 3 and serves as a carrier to transport caspase 3 from the cytoplasm to the nucleus to induce morphological changes in the nucleus.
UniGene ID
Hs.594496
UniProt ID (eCommerce)
O43823

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.