human AIFM1 shRNA silencing AAV

Name 
AAV-h-AIFM1-shRNA
Cat No 
shAAV-200620
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human AIFM1.

shAAV-200620
AAV-h-AIFM1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human AIFM1 (apoptosis inducing factor, mitochondria associated 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AIF; apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; striatal apoptosis-inducing factor; testicular secretory protein Li 4
Description (eCommerce)
Apoptosis-inducing factor (AIF) is a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, AIF induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
Description (Vector)
AIFM1 encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
Gene ID
9131
Gene Name (eCommerce)
apoptosis inducing factor mitochondria associated 1
Gene Name (Vector)
apoptosis inducing factor, mitochondria associated 1
Gene Symbol
AIFM1
HGNC ID
HGNC:8768
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1830
ORF Size (bp)
1830 bp
Protein Name (eCommerce)
Apoptosis-inducing factor 1, mitochondrial
RefSeq ID
NM_004208
RefSeq Synonyms
NM_145813, NM_145812, NM_004208, NM_001130847, NM_001130846, BC139738, BC111065,
Research Areas
Apoptosis,DNA Damage/Repair,Mitochondrion,Neurobiology,Neurodegeneration,Neurodevelopment,Signal Transduction
Research Areas (Faceted)
cell_biology,developmental_biology,genetics,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Apoptosis-inducing factor (AIF) is a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, AIF induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
UniGene ID
Hs.424932
UniProt ID (eCommerce)
O95831

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.