human ACY1 shRNA silencing AAV

Name
AAV-h-ACY1-shRNA

Cat No
shAAV-200334

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ACY1.

shAAV-200334

AAV-h-ACY1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACY1 (aminoacylase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ACY-1; ACY1D; HEL-S-5

Description (Vector)

ACY1 encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.

Gene ID

Gene Name (Vector)

aminoacylase 1

Gene Symbol

ACY1

HGNC ID

HGNC:177

ORF Size (aa)

1227

ORF Size (bp)

1227 bp

RefSeq ID

NM_000666

RefSeq Synonyms

NM_001198898, NM_001198897, NM_001198896, NM_001198895, NM_000666, BC014112, BC003023, BC000545,

Species

human

UniGene ID

Hs.334707

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.