human ACTN1 shRNA silencing AAV

Name
AAV-h-ACTN1-shRNA

Cat No
shAAV-200299

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ACTN1.

shAAV-200299

AAV-h-ACTN1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACTN1 (actinin alpha 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BDPLT15

Description (Vector)

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Gene ID

Gene Name (Vector)

actinin alpha 1

Gene Symbol

ACTN1

HGNC ID

HGNC:163

ORF Size (aa)

2679

ORF Size (bp)

2679 bp

RefSeq ID

NM_001102

RefSeq Synonyms

NM_001130005, NM_001130004, NM_001102, BC015766, BC003576,

Species

human

UniGene ID

Hs.509765

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.