mouse EMG1 shRNA silencing Adenovirus

Name 
Ad-m-EMG1-shRNA
Cat No 
shADV-258325
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Emg1.

shADV-258325
Ad-m-EMG1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Emg1 (EMG1 N1-specific pseudouridine methyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C2f; Grcc2f
Description (Vector)
Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position.
Gene ID
14791
Gene Name (Vector)
EMG1 N1-specific pseudouridine methyltransferase
Gene Symbol
Emg1
ORF Size (aa)
735
ORF Size (bp)
735 bp
RefSeq ID
NM_013536
RefSeq Synonyms
NM_013536, BC002004,
Species
mouse
UniGene ID
Mm.6390

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.