mouse EIF4A1 shRNA silencing Adenovirus

Name 
Ad-m-EIF4A1-shRNA
Cat No 
shADV-258225
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Eif4a1.

shADV-258225
Ad-m-EIF4A1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Eif4a1 (eukaryotic translation initiation factor 4A1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BM-010; Ddx2a; Eif4
Description (Vector)
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Gene ID
13681
Gene Name (Vector)
eukaryotic translation initiation factor 4A1
Gene Symbol
Eif4a1
ORF Size (aa)
1221
ORF Size (bp)
1221 bp
RefSeq ID
NM_144958
RefSeq Synonyms
NM_144958, NM_001159375, BC049915,
Species
mouse
UniGene ID
Mm.473799

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.