human RIC8A shRNA silencing Adenovirus

Name 
Ad-h-RIC8A-shRNA
Cat No 
shADV-221213
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RIC8A.

shADV-221213
Ad-h-RIC8A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RIC8A (RIC8 guanine nucleotide exchange factor A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Protein Ric-8A; resistance to inhibitors of cholinesterase 8 homolog A; RIC8; synembryn-A
Description (eCommerce)
RIC8 guanine nucleotide exchange factor A (Ric-8A), also known as Synmebryn-A, is a guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. It is able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Ric-8A is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). It also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NPQ8].
Gene ID
60626
Gene Name (eCommerce)
RIC8 guanine nucleotide exchange factor A
Gene Name (Vector)
RIC8 guanine nucleotide exchange factor A
Gene Symbol
RIC8A
HGNC ID
HGNC:29550
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1593
ORF Size (bp)
1593 bp
Protein Name (eCommerce)
Synembryn-A
RefSeq ID
NM_021932
RefSeq Synonyms
NM_021932, NM_001286134, BC121808, BC121807, BC111499, BC011821,
Research Areas
Neurobiology
Research Areas (Faceted)
neurobiology
Species
human
Target Sentence
RIC8 guanine nucleotide exchange factor A (Ric-8A), also known as Synmebryn-A, is a guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. It is able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Ric-8A is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). It also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NPQ8].
UniGene ID
Hs.592292
UniProt ID (eCommerce)
Q9NPQ8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.