human RFWD2 shRNA silencing Adenovirus

Name 
Ad-h-RFWD2-shRNA
Cat No 
shADV-221064
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RFWD2.

shADV-221064
Ad-h-RFWD2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RFWD2 (ring finger and WD repeat domain 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
constitutive photomorphogenesis protein 1 homolog; constitutive photomorphogenic protein 1; E3 ubiquitin-protein ligase COP1; E3 ubiquitin-protein ligase RFWD2; hCOP1; putative ubiquitin ligase COP1; RFWD2; ring finger and WD repeat domain 2, E3 ubiquitin protein ligase; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-type E3 ubiquitin transferase RFWD2; RNF200
Description (eCommerce)
Constitutive photomorphogenic protein 1 (COP1) has been characterized in plants as a RING-finger-containing protein that functions to repress plant photomorphogenesis. In mammals, COP1 has been identified as an E3 ubiquitin ligase that negatively regulates p53 protein levels in unstressed cells. In response to DNA damage COP1 is phosphorylated by the ataxia telangiectasia mutated (ATM) protein kinase and is rapidly degraded releasing p53 to exert its tumor suppressor activities.
Gene ID
64326
Gene Name (eCommerce)
COP1, E3 ubiquitin ligase
Gene Name (Vector)
ring finger and WD repeat domain 2
Gene Symbol
RFWD2
HGNC ID
HGNC:17440
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2196
ORF Size (bp)
2196 bp
Protein Name (eCommerce)
E3 ubiquitin-protein ligase RFWD2
RefSeq ID
NM_001001740
RefSeq Synonyms
NM_022457, NM_001286644, NM_001001740, BC094728,
Research Areas
Cancer,Cell Biology,Cell Cycle,Cytoskeleton,DNA Damage/Repair,p53 Signaling,Signal Transduction,Tumor Suppressors/Oncoproteins,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Constitutive photomorphogenic protein 1 (COP1) has been characterized in plants as a RING-finger-containing protein that functions to repress plant photomorphogenesis. In mammals, COP1 has been identified as an E3 ubiquitin ligase that negatively regulates p53 protein levels in unstressed cells. In response to DNA damage COP1 is phosphorylated by the ataxia telangiectasia mutated (ATM) protein kinase and is rapidly degraded releasing p53 to exert its tumor suppressor activities.
UniGene ID
Hs.523744
UniProt ID (eCommerce)
Q8NHY2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.