human RFC4 shRNA silencing Adenovirus

Name 
Ad-h-RFC4-shRNA
Cat No 
shADV-221039
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RFC4.

shADV-221039
Ad-h-RFC4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RFC4 (replication factor C subunit 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A1; A1 37 kDa subunit; activator 1 37 kDa subunit; activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; replication factor C 37 kDa subunit; replication factor C subunit 4; RFC 37 kDa subunit; RF-C 37 kDa subunit; RFC37
Description (eCommerce)
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5.
Description (Vector)
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Gene ID
5984
Gene Name (eCommerce)
replication factor C subunit 4
Gene Name (Vector)
replication factor C subunit 4
Gene Symbol
RFC4
HGNC ID
HGNC:9972
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1092
Protein Name (eCommerce)
Replication factor C subunit 4
RefSeq ID
NM_002916
RefSeq Synonyms
NM_181573, NM_002916, BC024022, BC017452,
Research Areas
Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Homologous Recombination,Replication,Signal Transduction,Telomeric Maintenance
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,genetics
Species
human
Target Sentence
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5.
UniGene ID
Hs.714318
UniProt ID (eCommerce)
P35249

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.