human PRPF3 shRNA silencing Adenovirus

Name 
Ad-h-PRPF3-shRNA
Cat No 
shADV-219950
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PRPF3.

shADV-219950
Ad-h-PRPF3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PRPF3 (pre-mRNA processing factor 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HPRP3; HPRP3P; pre-mRNA-splicing factor 3; PRP3; PRP3 pre-mRNA processing factor 3 homolog; Prp3p; RP18; SNRNP90; U4/U6 small nuclear ribonucleoprotein Prp3; U4/U6 snRNP 90 kDa protein; U4/U6-associated RNA splicing factor
Description (eCommerce)
hPrp3p (human pre-mRNA splicing factor 3) was identified as a factor that associated with U4/U6 snRNPs which are components of the splicesome. hPrp3p is homologous to S. cerevisiae splicing factor Prp3p. Mutations in the PRPF3 gene are associated with retinitis pigmentosa-18. It has been determined that mutations in hPrp3p may alter the recognition motif for phosphorylation by casein kinase II (CKII) and that loss of phosphorylation may result in aberrant interactions within the U4/U6 snRNP complex.
Description (Vector)
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.
Gene ID
9129
Gene Name (eCommerce)
pre-mRNA processing factor 3
Gene Name (Vector)
pre-mRNA processing factor 3
Gene Symbol
PRPF3
HGNC ID
HGNC:17348
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2052
ORF Size (bp)
2052 bp
Protein Name (eCommerce)
U4/U6 small nuclear ribonucleoprotein Prp3
RefSeq ID
NM_004698
RefSeq Synonyms
NM_004698, BC001954, BC000184,
Research Areas
Gene Expression,mRNA Processing,mRNA Splicing,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
hPrp3p (human pre-mRNA splicing factor 3) was identified as a factor that associated with U4/U6 snRNPs which are components of the splicesome. hPrp3p is homologous to S. cerevisiae splicing factor Prp3p. Mutations in the PRPF3 gene are associated with retinitis pigmentosa-18. It has been determined that mutations in hPrp3p may alter the recognition motif for phosphorylation by casein kinase II (CKII) and that loss of phosphorylation may result in aberrant interactions within the U4/U6 snRNP complex.
UniGene ID
Hs.11776
UniProt ID (eCommerce)
O43395

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.