mouse RBX1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Rbx1.
shAAV-270300
AAV-m-RBX1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Rbx1 (ring-box 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
1500002P15Rik; AA517855; ROC1
Description (Vector)
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
Gene ID
56438
Gene Name (Vector)
ring-box 1
Gene Symbol
Rbx1
ORF Size (aa)
327
RefSeq ID
NM_019712
RefSeq Synonyms
NM_019712, BC056992, BC051473, BC027396,
Species
mouse
UniGene ID
Mm.29405
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.