mouse EPHB3 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Ephb3.
shAAV-258580
AAV-m-EPHB3-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Ephb3 (Eph receptor B3). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AW456895; Cek10; Etk2; HEK2; MDK5; Sek4; Tyro6
Description (Vector)
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobs of the cerebral cortex. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Gene ID
13845
Gene Name (Vector)
Eph receptor B3
Gene Symbol
Ephb3
ORF Size (aa)
2982
ORF Size (bp)
2982 bp
RefSeq ID
NM_010143
RefSeq Synonyms
NM_010143, BC053085, BC014822,
Species
mouse
UniGene ID
Mm.6972
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.