mouse ENPP6 shRNA silencing AAV

Name 
AAV-m-ENPP6-shRNA
Cat No 
shAAV-258391
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Enpp6.

shAAV-258391
AAV-m-ENPP6-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Enpp6 (ectonucleotide pyrophosphatase/phosphodiesterase 6). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4833421B01Rik; B830047L21Rik; D8Ertd514e; E-NPP 6; GPC-Cpde; Npp6
Description (Vector)
Choline-specific glycerophosphodiester phosphodiesterase. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently.
Gene ID
320981
Gene Name (Vector)
ectonucleotide pyrophosphatase/phosphodiesterase 6
Gene Symbol
Enpp6
ORF Size (aa)
1323
ORF Size (bp)
1323 bp
RefSeq ID
NM_177304
RefSeq Synonyms
NM_177304, BC096376,
Species
mouse
UniGene ID
Mm.211429

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.