mouse EMG1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Emg1.
shAAV-258325
AAV-m-EMG1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Emg1 (EMG1 N1-specific pseudouridine methyltransferase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
C2f; Grcc2f
Description (Vector)
Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position.
Gene ID
14791
Gene Name (Vector)
EMG1 N1-specific pseudouridine methyltransferase
Gene Symbol
Emg1
ORF Size (aa)
735
ORF Size (bp)
735 bp
RefSeq ID
NM_013536
RefSeq Synonyms
NM_013536, BC002004,
Species
mouse
UniGene ID
Mm.6390
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.
