mouse EIF4A2 shRNA silencing AAV

Name 
AAV-m-EIF4A2-shRNA
Cat No 
shAAV-258226
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Eif4a2.

shAAV-258226
AAV-m-EIF4A2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Eif4a2 (eukaryotic translation initiation factor 4A2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4833432N07Rik; BM-010; Ddx2b; eIF-4A-II; Eif4; eIF4A-II
Description (Vector)
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Gene ID
13682
Gene Name (Vector)
eukaryotic translation initiation factor 4A2
Gene Symbol
Eif4a2
ORF Size (aa)
1224
ORF Size (bp)
1224 bp
RefSeq ID
NM_013506
RefSeq Synonyms
NM_013506, NM_001347179, NM_001123038, NM_001123037, BC094422,
Species
mouse
UniGene ID
Mm.260084

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.