human SART1 shRNA silencing AAV

Name 
AAV-h-SART1-shRNA
Cat No 
shAAV-222262
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SART1.

shAAV-222262
AAV-h-SART1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SART1 (squamous cell carcinoma antigen recognized by T-cells 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
allergen Hom s 1; Ara1; HAF; HOMS1; hSART-1; hSnu66; hypoxia associated factor; IgE autoantigen; SART-1; SART1(259) protein; SART1(800) protein; SART1, U4/U6.U5 tri-snRNP-associated protein 1; SART1259; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNRNP110; Snu66; SNU66 homolog; squamous cell carcinoma antigen recognised by T cells; squamous cell carcinoma antigen recognized by T-cells 1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1
Description (eCommerce)
SART1 (squamous cell carcinoma antigen recognized by T cells 1) was originally identified as a gene that encodes an antigenic peptide expressed in squamous cell carcinomas and recognized by cytotoxic T lymphocytes. The SART1 gene encodes two proteins, SART1(800) and SART1(259). SART1(800) has been found to be expressed in the nucleus of the majority of proliferating cells and has been identified as a component of the splicesomal complex required for U4/U6.U5 tri-snRNP recruitment and the assembly of mature splicesomes.
Description (Vector)
SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.
Gene ID
9092
Gene Name (eCommerce)
spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP
Gene Name (Vector)
squamous cell carcinoma antigen recognized by T-cells 1
Gene Symbol
SART1
HGNC ID
HGNC:10538
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2403
ORF Size (bp)
2403 bp
Protein Name (eCommerce)
U4/U6.U5 tri-snRNP-associated protein 1
RefSeq ID
NM_005146
RefSeq Synonyms
NM_005146, BC001058,
Research Areas
Apoptosis,Cell Cycle,Gene Expression,Golgi Apparatus,Immunology,mRNA Processing,mRNA Splicing,RNA Processing,Spliceosome
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,cell_biology,immunology
Species
human
Target Sentence
SART1 (squamous cell carcinoma antigen recognized by T cells 1) was originally identified as a gene that encodes an antigenic peptide expressed in squamous cell carcinomas and recognized by cytotoxic T lymphocytes. The SART1 gene encodes two proteins, SART1(800) and SART1(259). SART1(800) has been found to be expressed in the nucleus of the majority of proliferating cells and has been identified as a component of the splicesomal complex required for U4/U6.U5 tri-snRNP recruitment and the assembly of mature splicesomes.
UniGene ID
Hs.502883
UniProt ID (eCommerce)
O43290

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.