human RPP25 shRNA silencing AAV

Name
AAV-h-RPP25-shRNA

Cat No
shAAV-221794

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RPP25.

shAAV-221794

AAV-h-RPP25-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RPP25 (ribonuclease P/MRP subunit p25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ribonuclease P 25kDa subunit; ribonuclease P protein subunit p25; ribonuclease P/MRP 25kDa subunit; ribonuclease P/MRP subunit p25; RNase P protein subunit p25

Description (eCommerce)

RPP25 encodes Ribonuclease P protein subunit p25. Ribonuclease P is a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P protein subunit p25 is also a component of RNase MRP. This subunit binds to RNA [taken from UniProtKB (Entry: Q9BUL9)].

Gene ID

54913

Gene Name (eCommerce)

ribonuclease P and MRP subunit p25

Gene Name (Vector)

ribonuclease P/MRP subunit p25

Gene Symbol

RPP25

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

600

ORF Size (bp)

600 bp

Protein Name (eCommerce)

Ribonuclease P protein subunit p25

RefSeq ID

NM_017793

RefSeq Synonyms

NM_017793, BC007270, BC002497,

Research Areas

Mitochondrion,RNA Binding,RNA Processing,RNA Transport

Research Areas (Faceted)

cell_biology,genetics,transcription_translation

Species

human

Target Sentence

RPP25 encodes Ribonuclease P protein subunit p25. Ribonuclease P is a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P protein subunit p25 is also a component of RNase MRP. This subunit binds to RNA [taken from UniProtKB (Entry: Q9BUL9)].

UniGene ID

Hs.8562

UniProt ID (eCommerce)

Q9BUL9

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.