human RNASE1 shRNA silencing AAV

Name
AAV-h-RNASE1-shRNA

Cat No
shAAV-221286

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RNASE1.

shAAV-221286

AAV-h-RNASE1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RNASE1 (ribonuclease A family member 1, pancreatic). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

RAC1; RIB1; RNS1

Description (Vector)

RNASE1 encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Gene ID

6035

Gene Name (Vector)

ribonuclease A family member 1, pancreatic

Gene Symbol

RNASE1

HGNC ID

HGNC:10044

ORF Size (aa)

471

RefSeq ID

NM_002933

RefSeq Synonyms

NM_198235, NM_198234, NM_198232, NM_002933, BC022882, BC005324,

Species

human

UniGene ID

Hs.78224

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.