human RFK shRNA silencing AAV

Name
AAV-h-RFK-shRNA

Cat No
shAAV-221047

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human RFK.

shAAV-221047

AAV-h-RFK-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RFK (riboflavin kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

RIFK

Description (Vector)

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 (PubMed 12623014)).(supplied by OMIM, Nov 2009)

Gene ID

55312

Gene Name (Vector)

riboflavin kinase

Gene Symbol

RFK

HGNC ID

HGNC:30324

ORF Size (aa)

489

RefSeq ID

NM_018339

RefSeq Synonyms

NM_018339, BC007069,

Species

human

UniGene ID

Hs.37558

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.