human PRPF31 shRNA silencing AAV

Name
AAV-h-PRPF31-shRNA

Cat No
shAAV-219952

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PRPF31.

shAAV-219952

AAV-h-PRPF31-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRPF31 (pre-mRNA processing factor 31). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

hPrp31; NY-BR-99; Pre-mRNA-processing factor 31; protein 61K; PRP31; PRP31 pre-mRNA processing factor 31 homolog; RP11; serologically defined breast cancer antigen NY-BR-99; SNRNP61; U4/U6 small nuclear ribonucleoprotein Prp31; U4/U6 snRNP 61 kDa protein

Description (eCommerce)

Pre-mRNA-processing factor 31 (PRPF31) is involved in pre-mRNA splicing and is required for U4/U6.U5 tri-snRNP formation. Defects in the PRPF31 gene are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8WWY3].

Description (Vector)

PRPF31 encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.

Gene ID

26121

Gene Name (eCommerce)

pre-mRNA processing factor 31

Gene Name (Vector)

pre-mRNA processing factor 31

Gene Symbol

PRPF31

HGNC ID

HGNC:15446

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1500

ORF Size (bp)

1500 bp

Protein Name (eCommerce)

U4/U6 small nuclear ribonucleoprotein Prp31

RefSeq ID

NM_015629

RefSeq Synonyms

NM_015629, BC117389,

Research Areas

Gene Expression,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome

Research Areas (Faceted)

genetics,transcription_translation,cell_biology

Species

human

Target Sentence

Pre-mRNA-processing factor 31 (PRPF31) is involved in pre-mRNA splicing and is required for U4/U6.U5 tri-snRNP formation. Defects in the PRPF31 gene are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8WWY3].

UniGene ID

Hs.515598

UniProt ID (eCommerce)

Q8WWY3

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.