human EHMT1 shRNA silencing AAV

Name 
AAV-h-EHMT1-shRNA
Cat No 
shAAV-207688
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human EHMT1.

shAAV-207688
AAV-h-EHMT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human EHMT1 (euchromatic histone lysine methyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
EHMT1 intronic transcript 1; EHMT1-IT1; euchromatic histone-lysine N-methyltransferase 1; EUHMTASE1; Eu-HMTase1; FP13812; G9a-like protein 1; GLP; GLP1; H3-K9-HMTase 5; histone H3-K9 methyltransferase 5; histone-lysine N-methyltransferase EHMT1; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; KLEFS1; KMT1D; lysine N-methyltransferase 1D
Description (eCommerce)
Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is a histone methyltransferase that catalyzes the methylation of lysine-9 of histone H3 (H3-K9). H3-K9 histone methylation is restricted to euchromatin and functions to epigenetically silence gene transcription. Loss of function mutations in EHMT1 cause the 9q34 subtelomeric deletion syndrome, a syndrome characterized by severe mental retardation, hypotonia, brachy(micro)cephaly, heart defects, and distinct facial anomalies.
Description (Vector)
EHMT1 is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome). Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
79813
Gene Name (eCommerce)
euchromatic histone lysine methyltransferase 1
Gene Name (Vector)
euchromatic histone lysine methyltransferase 1
Gene Symbol
EHMT1
HGNC ID
HGNC:24650
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2427
ORF Size (bp)
2427 bp
Protein Name (eCommerce)
Histone-lysine N-methyltransferase EHMT1
RefSeq ID
NM_024757
RefSeq Synonyms
NM_024757, NM_001145527, BC047504,
Research Areas
Chromatin Remodeling,Histone Modification,Immunology,Methyltransferase,Neurobiology
Research Areas (Faceted)
cell_biology,genetics,immunology,neurobiology
Species
human
Target Sentence
Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is a histone methyltransferase that catalyzes the methylation of lysine-9 of histone H3 (H3-K9). H3-K9 histone methylation is restricted to euchromatin and functions to epigenetically silence gene transcription. Loss of function mutations in EHMT1 cause the 9q34 subtelomeric deletion syndrome, a syndrome characterized by severe mental retardation, hypotonia, brachy(micro)cephaly, heart defects, and distinct facial anomalies.
UniGene ID
Hs.495511
UniProt ID (eCommerce)
Q9H9B1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.