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AAV ITR Vectors

We have developed over 15 different AAV ITR plasmids for AAV production. All vectors here contains the L-ITR and R-ITR from AAV serotype 2, and it's compatible with Helper Free AAV Systems.

Here are the most commonly used AAV ITR vectors. To get any of the plasmids listed here, please request it online or send us an email.

AAV Vectors for Gene Over-expression

  • pAAV-BASIC * (map): empty ITR vector, for cloning of your whole existing cassette
  • pAAV-BASIC-EGFP * (map): Same as pAAV-BASIC, but with co-expression of eGFP.

  • pAAV-cDNA6-V5His (map): with CMV promoter, for cloning of cDNA only.

  • pAAV-GFP-cDNA6 (map): same as pAAV-cDNA6-V5His, but also co-expressing GFP.



AAV ITR vectors for shRNA/miRNA

    For cloning from an existing shRNA/miRNA expression cassette:

  • pAAV-BASIC * (map): empty ITR vector, for cloning of your whole existing cassette
  • pAAV-BASIC-EGFP * (map): Same as pAAV-BASIC, but with co-expression of eGFP.


    For cloning from an shRNA oligos

  • pAAV-U6 (map): the shRNA expression will be under the control of U6-promoter in this vector.

  • pAAV-U6-EGFP (map): same as pAAV-U6, but also co-express eGFP as marker

  • pAAV-Siln41-MCS (map): shRNA expression will be under the control of a modified CMV promoter.

  • pAAV-Siln41-MCS-EGFP (map): same as pAAV-Siln41-MCS, but also co-express eGFP as marker


* We also have the same vector with RFP co-expression instead of GFP.

If you don't the see the vectors you need here, please email us for additional AAV vector selections not listed here.


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